Table 3.
Proteins interacted with FLAG-human FABP7wt and without FLAG-human FABP7mut from HEK293 cells
| Gene name | Protein name | Protein score1 | Spectral count2 | Sequence3 | Spectral count in mock4 | Spectral count in FLAG-human FABP7mut5 |
|---|---|---|---|---|---|---|
| FLAG-humanFABP7 | 158,094 | 4827 | 31 | 6 | 1519 | |
| PKP1 | Plakophilin-1 | 828 | 31 | 7 | – | 3 |
| LGALS7 | Galectin-7 | 423 | 11 | 4 | – | – |
| EPPK1 | Epiplakin | 312 | 17 | 12 | – | – |
| SERPINB3 | Serpin B3 | 295 | 14 | 4 | – | – |
| SERPINB4 | Serpin B4 | 291 | 16 | 5 | – | – |
| ACTN4 | Alpha-actinin-4 | 287 | 13 | 5 | – | – |
| HNRNPC | Heterogeneous nuclear ribonucleoproteins C1/C2 | 256 | 6 | 2 | – | – |
| ATP5B | ATP synthase subunit beta, mitochondrial | 255 | 22 | 5 | 1 | 1 |
| TUBA1A | Tubulin alpha-1A chain | 246 | 8 | 5 | – | 1 |
| HIST1H2BB | Histone H2B type 1-B | 219 | 7 | 3 | – | – |
| HSPB1 | Heat shock protein beta-1 | 188 | 9 | 6 | – | – |
| SFN | 14-3-3 protein sigma | 166 | 10 | 4 | – | – |
| PLP2 | Proteolipid protein 2 | 157 | 7 | 1 | – | – |
| PARP1 | Poly [ADP-ribose] polymerase 1 | 148 | 4 | 2 | – | – |
| GSN | Gelsolin | 144 | 8 | 5 | – | – |
| TGM1 | Protein-glutamine gamma-glutamyltransferase K | 111 | 3 | 1 | – | – |
| CAPG | Macrophage-capping protein | 96 | 5 | 2 | – | – |
| ACLY | ATP-citrate synthase | 83 | 3 | 1 | – | – |
| TYMP | Thymidine phosphorylase | 75 | 4 | 2 | – | – |
| DSG3 | Desmoglein-3 | 67 | 2 | 2 | – | – |
Shown are representative proteins interacted with FABP7wt and without FABP7mut, with their MASCOT protein scores, spectral counts, and number of sequence. False discovery rate (FDR) calculated by MASCOT decoy search was 3.10%
1Protein score was determined by the MASCOT software
2Number of uniquely assigned spectral count for the respective proteins is shown
3Number of sequence match for the respective proteins is shown
4Number of spectral count for the respective proteins in the mock purification is shown
5Number of spectral count for the respective proteins in the FLAG-FABP7mut purification is shown