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. Author manuscript; available in PMC: 2020 Oct 27.
Published in final edited form as: Nat Biotechnol. 2020 Apr 27;38(10):1194–1202. doi: 10.1038/s41587-020-0505-4

Figure 5. Antigen discovery for TCR specificity group I.

Figure 5.

a, Antigen screen for TCR121 as described in figure 3. b, Individual protein from the four positive subpools (a) were expressed separately and screened against TCR121. Mean ± s.d. (n=2, biological replicates) shown. c, The five PPE proteins identified from (b) were analyzed by NetMHCIIpan. Top ranked peptides with high binding affinity to DRB3*0301 were listed. d-e, Dose-dependent response of Group I TCR121 (d) and TCR122 (e) to the top ranked peptides. Mean ± s.d. (n=3, biological replicates) shown. f, EC50 values were determined as described in figure 3.