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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Kidney Int. 2020 Apr 25;98(4):897–905. doi: 10.1016/j.kint.2020.03.037

Figure 5. T cell depletion prevents upregulation of LCN2 in urine.

Figure 5.

(A) Mice were treated with antibodies to CD4 (GK1.5 and YTS191), CD8 (53-6.2 and 169.4) or a combination of both at days 0 and 3 after transplantation in the early treatment protocol for blocking PD-L1. (B) Depletion of T cells was confirmed by flow cytometry. Gated on CD45+ 7AAD− cells, circulating CD4 and CD8 T cells in the blood were analyzed at posttransplant day 6. (C) Absolute numbers of infiltrating CD4 and CD8 T cells were analyzed in the grafts at posttransplant day 6. (D) Urinary LCN2 was measured at days 3, 4, 5 and 6 by ELISA. (E) Blood urea nitrogen performed on serum at 6-7 days after transplantation. Each point represents an individual sample. Bars represent mean ± SEM. *P < 0.05, **P < 0.01 by 2-way ANOVA with Dunnett’s multiple comparisons test.