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. 2020 Oct 7;11:5045. doi: 10.1038/s41467-020-18813-x

Fig. 3. In vivo biofluorination in engineered P. putida.

Fig. 3

a Scheme of the FRS-T7 RNA polymerase circuit controlling the expression of the two fluorinases flA1 and SxflA in plasmid modules. b Growth curves of P. putida KT2440::FRS-T7RNAP containing the plasmids described in (a), compared to the growth of P. putida KT2440::FRS-T7RNAP transformed with an empty vector (pSEVA231). The timing of F− addition to the cultures is indicated with a black arrow. Green arrows indicate the points when cultures were harvested to test 5′-FDA biosynthesis. Mean values and standard deviations of the specific growth rates (μ) after the addition of F− of each construction are shown in the plot. Error bars correspond to standard deviations of three biological replicates. c Intracellular 5′-FDA concentration after 2, 20, 24, and 48 h of induction of the synthetic circuit with NaF. Data are presented as mean values and error bars correspond to standard deviations of three different biological replicates. CDW cell dry weight. Source data underlying Fig. 3b, c are provided as a Source Data file.