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. 2020 Oct 7;10:16754. doi: 10.1038/s41598-020-74011-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Suppression of NO production impairs smooth muscle cell development and recruitment. (A) IHC staining against smooth muscle α-actin (SM α-actin) to visualize the vascular smooth muscle cell development at E18.5. (B) Relative expression of SM α-actin in A quantified which based on the IOD (n = 4 mice per group, unpaired t-test). IHC staining against SM α-actin at E20.5 and quantification displayed in (C,D) (n = 4 mice per group, unpaired t-test). IHC staining against MHC at E20.5 and quantification displayed in (E,F) (n = 4 mice per group, unpaired t-test). Scale bar: 60 μm. Data presented as means ± SEM, * P < 0.05.