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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Cell Stem Cell. 2020 Aug 17;27(4):574–589.e8. doi: 10.1016/j.stem.2020.07.015

Figure 1. HLHS de novo mutations (DNMs) were enriched in the endocardium and endothelium based on scRNA-seq analysis of human fetal heart.

Figure 1.

(A) Schematic of the workflow for micro-dissection of normal human fetal heart and scRNA-seq. Endocardial/endothelial populations were enriched with a CD144 antibody and Magnetic-Activated Cell Sorting. PA: pulmonary artery; RA: right atrium; LA: left atrium; RV: right ventricle; LV: left ventricle. (B) UMAP projection of various cell types from day 83 normal human fetal heart. SMC: smooth muscle cell; RBC: red blood cell; CM: cardiomyocyte; EC: endothelial cell. (C) UMAP projection of represented genes for various cell types colored by represented genes’ expression level (purple indicates high expression level) in human fetal heart. (D) Cell type-specific RNA expression of HLHS DNM genes based on scRNA-seq from day 83 normal fetal heart. Row Z-score indicates RNA level. Red denotes high expression, blue minimal expression. See also Figure S1.