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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Cell Stem Cell. 2020 Aug 17;27(4):574–589.e8. doi: 10.1016/j.stem.2020.07.015

Figure 4. HLHS iEECs impeded cardiomyocyte proliferation and maturation.

Figure 4.

(A) Normal iPSC-CMs were co-cultured with iPSC-ECs from control or HLHS patients for 48 hr. (B) Transcriptomic profiling and functional enrichment analysis were performed on iPSC-CMs from (A) by bulk RNA-seq (n=3). −log10P indicates the significance of enrichment. Z-score defines the changing trend of enriched functions between HLHS vs control; z-score<0 means down-regulation in iPSC-CMs co-cocultured with HLHS iPSC-ECs. (C) Heatmap visualization of DEGs from GO terms in (B). (D) Immunostaining of Ki67 and TNNT2 in D15 iPSC-CMs co-cultured with control or HLHS iEECs for 48 hr. DAPI: nucleus; Green: Ki67 positive nucleus; Red: TNNT2 positive cardiomyocytes. (E) Gene expression related to proliferation in iPSC-CMs from (D). Contraction velocity (F), sarcomere organization (G), and maturation related gene expressions (H) in D30 iPSC-CMs co-cultured with control or HLHS iEECs. In (D-H), data shown as the mean ± SEM. *p<0.05, **p<0.01. See also Figure S3.