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. 2020 Oct 7;10:16680. doi: 10.1038/s41598-020-73497-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal microscopy of green fluorescence images of ROS in Cu₂O NPs treated, and untreated control cells measured by 2,7-dichlorofluorescin diacetate (DCFH-DA) fluorescence-based assay. Cells were treated with 0, 62.5 and 125 μg/mL of surfactant stabilized Cu₂O NPs for 24 h. (a–c) green fluorescence images of 0, 62.5 and 125 μg/mL dose Cu₂O NPs exposed Gram-positive B. subtilis CN2 cells. (d–f) green fluorescence images of 0, 62.5 and 125 μg/mL dose Cu₂O NPs exposed Gram-negative P. aeruginosa CB1 cells. Untreated cells were used as a negative control (a, d). Scale Bar: 5 µm.