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. 2020 Oct 7;10:16686. doi: 10.1038/s41598-020-73840-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mammalian somatotrophs express both NUCB1 and NUCB2. (a) Representative gel images showing nucb1, nucb2 and β-actin PCR products from both GH3 and RC-4B/C rat somatotroph cells. NTC: No Template Control. RTC: No Reverse Transcriptase Control. PCRC: PCR Control (No Template Control in the PCR). (b) Representative immunoreactive bands of NUCB1, NUCB2 and β-actin analyzed by Western blot in protein extracts from either GH3 or RC-4B/C rat somatotroph cells. (c) Representative images of NUCB1 and NUCB2 (in green) protein expression in GH3 cells counterstained with DAPI (in blue) and detected by immunofluorescence. The images below correspond to an amplification of the area indicated with an arrow in the corresponding image above. Images were acquired at 40X magnification.