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. 2020 Oct 7;10:16686. doi: 10.1038/s41598-020-73840-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NESF or NLP treatment decreases GH protein in mammalian somatotroph cells. Effects of 1 h incubation with NESF (a) or NLP (c), or of 6 h incubation (b and d, respectively), on the GH protein levels in GH3 cells detected by Western blot. Representative immunoreactive bands and quantification of GH band intensity. Four independent experiments with triplicates (n = 3 wells/treatment/experiment) were performed for each study. Data from all four experiments were pooled to conduct statistical analyses and are shown as mean ± SEM (n = 12 wells) normalized to the levels of β-actin and presented as a fold change over Control. Different letters indicate significant differences (p < 0.05) between the different concentrations detected by one-way ANOVA test followed by Tukey’s multiple comparison test.