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. 2020 Oct 7;10:16679. doi: 10.1038/s41598-020-73845-z

Figure 4.

Figure 4

FRZB immunoreactivity is increased in atrophic myofibers of ALS muscle samples. Muscle sections from ALS patients were immunostained for quantification of FRZB punctae. (A) Representative section (from a deltoid muscle) showing the presence of both atrophic (< 25 µM) and non-atrophic myofibers (> 25 µM). The section is also immunostained with an FRZB antibody. (B) Micrographs were analyzed by ImageJ where the number of FRZB punctae associated with non-atrophic and atrophic myofibers was quantitated (yellow tracing shown in the representative section). (C) Quantitative results of FRZB-positive punctae in muscle sections from 5 ALS patients (1 deltoid, 1 biceps and 3 vastus lateralis muscles). Columns represent the mean ± SEM of 34 atrophic and 26 non-atrophic fibers analyzed. **P = 0.009. Scale bars, 50 μM.