Table 2.
Differentially expressed proteins proteins significantly more abundant in rabbit myofibroblasts from cornea than from BM.
| Rabbit accession UniProt | Human accession UniProt | Gene symbol | Protein | Sample frequency | Linear ratio cornea/bone marrow | % identity rabbit and human proteins | Adjusted p valueD |
|---|---|---|---|---|---|---|---|
| G1SIW5 | Q92820 | GGH | Folate gamma-glutamyl hydrolase | 4 | 4.78 | 82 | 3.5E−04 |
| G1U4P8 | Q9BXN1 | ASPN | Asporin | 4 | 3.05 | 88 | 3.5E−04 |
| G1SDA2 | P29762 | CRABP1 | FABP domain-containCellular retinoic acid-binding protein 1ing protein | 4 | 2.89 | 99 | 1.7E−04 |
| G1TW43 | J3QSU6 | TNC | Tenascin | 4 | 2.73 | 74 | 1.7 E−04 |
| G1ST69 | P20700 | LMNB1 | Lamin-B1 | 4 | 2.73 | 98 | 1.7 E−04 |
| G1T380 | Q02388 | COL7A1 | Collagen alpha-1(VII) chain | 4 | 2.73 | 87 | 1.6 E−03 |
| G1SJF4 | A0A0A0MR51 | FADS1 | Acyl-CoA (8–3)-desaturase | 4 | 2.66 | 93 | 4.7E−04 |
| G1T3Y8 | P10809 | HSPD1 | 60 kDa heat shock protein, mitochondrial | 4 | 2.66 | 99 | 1.7E−04 |
| G1SJW7 | J3QLE5 | SNRPN | Small nuclear ribonucleoprotein-associated protein | 4 | 2.57 | 100 | 1.7E−04 |
| G1SK42 | P21980 | TGM2 | Protein-glutamine gamma-glutamyltransferase 2 | 4 | 2.55 | 87 | 2.8E−04 |
| G1SEF5 | Q8NAV1 | PRPF38A | Pre-mRNA-splicing factor 38A | 4 | 2.52 | 100 | 3.3E−04 |
| G1SNX5 | B4DY09 | ILF2 | Interleukin enhancer-binding factor 2 | 4 | 2.45 | 100 | 1.7E−04 |
| G1T2X0 | Q99541 | PLIN2 | Perilipin-2 | 4 | 2.45 | 88 | 2.6E−03 |
| G1SI79 | P51991 | HNRNPA3 | Heterogeneous nuclear ribonucleoprotein A3 | 4 | 2.44 | 100 | 1.7E−04 |
| G1SFC6 | A0A087WV29 | NAT10 | RNA cytidine acetyltransferase | 4 | 2.41 | 96 | 2.2E−04 |
| G1TD26 | D6RAA6 | TMEM33 | Transmembrane protein 33 (Fragment) | 4 | 2.40 | 99 | 1.7E−04 |
| G1SUP9 | P46087 | NOP2 | Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase | 4 | 2.40 | 77 | 1.9E−04 |
| P00389 | P16435 | POR | NADPH–cytochrome P450 reductase | 4 | 2.37 | 92 | 2.5E−04 |
| G1TLW3 | J3KTA4 | DDX5 | Probable ATP-dependent RNA helicase DDX5 | 4 | 2.35 | 96 | 1.7E−04 |
| G1SDW8 | H0Y2P0 | CD44 | CD44 antigen (Fragment) | 4 | 2.34 | 91 | 5.2E−04 |
| G1T4M2 | O60264 | SMARCA5 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 | 4 | 2.321 | 100 | 2.5E−04 |
| G1T2K5 | I3L1L3 | MYBBP1A | Myb-binding protein 1A (Fragment) | 4 | 2.318 | 69 | 1.9E−04 |
| G1SI26 | Q4VC31 | CCDC58 | Coiled-coil domain-containing protein 58 | 4 | 2.277 | 95 | 1.7E−04 |
| G1TX84 | Q9Y5J1 | UTP18 | U3 small nucleolar RNA-associated protein 18 homolog | 4 | 2.269 | 89 | 1.7E−04 |
| G1SMB3 | Q9NV31 | IMP3 | U3 small nucleolar ribonucleoprotein protein IMP3 | 4 | 2.260 | 99 | 1.7E−04 |
| G1SE74 | Q7KZ85 | SUPT6H | Transcription elongation factor SPT6 | 4 | 2.247 | 99 | 2.8E−04 |
| G1T3Y0 | P98082 | DAB2 | Disabled homolog 2 | 4 | 2.233 | 90 | 3.3E−04 |
| G1SIJ6 | Q13308 | PTK7 | Inactive tyrosine-protein kinase 7 | 4 | 2.232 | 93 | 1.7E−04 |
| G1TD41 | G8JLB6 | HNRNPH1 | Heterogeneous nuclear ribonucleoprotein H | 4 | 2.226 | 98 | 1.7E−04 |
| G1T6T0 | J3KNJ3 | NAALAD2 | N-acetylated-alpha-linked acidic dipeptidase 2 | 4 | 2.215 | 89 | 1.9E−04 |
The above 30 proteins were selected from 360 differentially expressed proteins more abundant in rabbit myofibroblasts from cornea than from BM. Each exhibited a protein ratio ≥ 1 SD from the mean and an adjusted pvalues ≤ 0.05 in ≥ 3 paired myofibroblast samples. All differentially expressed proteins are illustrated in Fig. 2 and identified in Supplemental Table 5.