Figure 1.

Semi-automated quantification of punctate hyperreflective vitreous opacities to determine Vitreous Opacity Ratio. For each right eye imaging session, each of five (A) B-scans centered on the fovea (total width ~256 microns) were analyzed by two trained graders through a custom web-based application to (B) manually circle punctate hyperreflective vitreous opacities to allow for computer counting of opacities and (C) manually demarcate the inner retinal border to allow for computer segmentation of the total vitreous area above the border. The VOR was calculated as the total number of punctate hyperreflective vitreous opacities among all five B-scans divided by the total visible vitreous area in pixels and averaged between the two graders. VOR = Vitreous Opacity Ratio.