Immunohistochemistry (IHC) analysis of β-catenin at the treatment area. (a) Representative images from different treatment groups of IHC staining of β-catenin of connective tissue above the alveolar bone crest (ABC) and between first molar (M1) and second molar (M2). (b) Semi-quantitative assessment of β-catenin positive cells interproximally between first molar (M1) and second molar (M2). Values are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. ###P < 0.001 when compared to Con+ group. (one-way ANOVA). Scale bar = 200 μm. Brown color indicates β-catenin staining, black arrows indicate alveolar bone, and asterisks indicate first molar (M1) and second molar (M2).