Figure 3.
Effect of 1 on cell viability in HS578T spheroids: (a) Normalized LDH release as a function of time and concentration of 1; (b-e) Cell death shown by microscopy images after (b, d) live/dead (projection of the whole spheroid) and (c, e) hematoxylin/eosin staining. LDH release of HS578T spheroids as a function of (f) ALP inhibition (I), (g) GLUT1 and (h) CAV1 knockdown (KD). The applied treatment (Treat.) was spheroid exposition to 1 (1 mM, 48 h). Statistics: ns (non-significant); * p < 0.01; ** p < 0.005; *** p < 0.001; **** p < 0.0001.