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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Cancer Res. 2020 Jul 2;80(19):4046–4057. doi: 10.1158/0008-5472.CAN-19-4032

Figure 3: Linc-SPRY3 RNAs are important for cell survival following 8Gy irradiation.

Figure 3:

(A) RT-PCR analysis shows expression of the linc-SPRY3 family in BAC RP11-88F4 nucleofected A549 cells. (B-C) Overall cell viability and apoptosis were measured by flow cytometry. Y chromosome BAC nucleofection results in lower cell viability and increased apoptosis compared to control in radioresistant A549 cells after 8Gy IR. (D, G) qRT-PCR analysis reveals effective knockdown of the linc-SPRY3 RNAs in radiosensitive cell lines H460 and WVU-Ma-0005. (E-F, H-I) Knockdown of the linc-SPRY3 family in both H460 and WVU-Ma-0005 cells using two different shRNAs against linc-SPRY3 family (sh1 and sh2) resulted in better overall cell viability and increased resistance to apoptosis when compared with shControl (shCtrl) after 8Gy IR. Untreated controls are provided to demonstrate baseline cell viability and apoptosis. (J-K) Tumor growth delay assay (n = 3) shows higher resistance to radiation of the sh1 tumors relative to shCtrl tumors in both H460 and WVU-Ma-0005 animal experiments. Mean relative tumor volumes are plotted. Error bars represent SD from the mean (n=3) (A-I). Error bars represent SD from the mean of 3 mice in each group (J-K). Untreated tumor controls are represented in Supplementary Fig. S9. ANOVA was used to determine statistical significance.