Figure 5. GVAX + IPI promotes an immunostimulatory phenotype in the metastatic pancreatic tumor microenvironment.

A. Longitudinal changes of immune cell composition of CD45⁺ leukocyte cell densities from baseline (blue circle) to week 7 GVAX + IPI (red square). P values <0.05 indicates statistical significance; Student’s t-tests. B-D. Image visualization by pseudocoloring of baseline and on-treatment FFPE sections of two representative areas in metastatic pancreatic tumor biopsies taken from one Arm A patient who exhibited stable disease. FFPE sections are stained via Multiplex IHC for (B) CD45⁺CD4⁺ T helper cells (red arrow), CD45⁺CD8⁺ cytotoxic T cells (blue arrow), (C) CD45⁺CD4⁺Foxp3⁺ T regulatory cells (blue arrow), (D) CD45⁺CSF1R⁺CD68⁺CD163⁺ M2 macrophages (red arrow), and CD45⁺CSF1R⁺CD68⁺CD163⁻ M1 macrophages (blue arrow). E. Comparative analysis of CD8⁺ T cell functional status at baseline and on-treatment.