Figure 3.

Itch does not regulate CD4 T cell metabolism during initial activation. Naïve T cells were isolated and rested in IL-2 for 24 h (A) and (C) or activated using IL-2 and anti-CD3 and anti-CD28 beads for 24 h (B) and (D). After this time, cells in (A) and (B) were tested for basal glycolysis and glycolytic activity by measuring extracellular acidification rate (ECAR) during treatment with the indicated drugs, including glucose, oligomycin, 2-deoxy-D-Glucose (2DG), and/or carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) with rotenone, using a seahorse assay. Cells in (C) and (D) were tested for basal oxidative phosphorylation and oxidative phosphorylation capacity by measuring oxygen consumption rate (OCR) during treatment with the indicated drugs, using a seahorse assay. n = 5 mice for restimulated cells (C) and (D) in three independent experiments, and n = 4 mice for unstimulated cells (A) and (B) across three independent experiments.