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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Cancer Res. 2020 Jul 14;80(19):4288–4301. doi: 10.1158/0008-5472.CAN-19-1564

Figure 5. Intravasation of a tumor cluster following mosaic vessel formation.

Figure 5.

Frames from a representative (A) phase contrast and (B) fluorescence time-lapse movie of a ROSAmTmG; MMTV-PyMT tumor organoid and a HUVEC microvessel (GFP). Insets of phase (A’) and fluorescent (B’) time-lapse images showing a tumor cluster shedding into the vessel lumen under continuous flow (yellow arrows). The flow rate is kept at 1mL/hour and shear stress at 4 dynes/cm2. Direction of flow is from left to right. Scale bars: 100 μm.