Table 1:

Immune Conditioning Regimens. All animals received 7 Gy of Thymic Irradiation on Day −1; 50 mg/kg of ATGAM on Days −2, −1 and 0; and 28-days of Cyclosporine A with a target trough level of 300–400 ng/mL. Total Body Irradiation of 1.5 Gy per dose was given on POD −7 and −6 if x 2, or only on POD −6 if x 1

Protocol Group	Recipient Animals	Total Body Irradiation (Gy)	Thymic Irradiation (Gy)	T-Cell Depletion (ATGAM)	Cyclosporine A	Anti-CD154 mAh or Splenectomy	Rituximab
A	90–57	1.5 × 2	7	50 mg/kg Day −2, −1, 0	Days 0–28	Anti-CD154 mAb	No
B	90–12 90–166	1.5 × 2	7	50 mg/kg Day −2, −1, 0	Days 0–28	Splenectomy	No
C	GA704C GR677K	1.5 × 1	7	50 mg/kg Day −2, −1, 0	Days 0–28	Splenectomy	No
D	N770L SA110B	1.5 × 1	7	50 mg/kg Day −2, −1, 0	Days 0–28	Splenectomy	20 mg/kg Day −6