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. Author manuscript; available in PMC: 2021 Oct 15.
Published in final edited form as: J Immunol. 2020 Sep 2;205(8):2128–2136. doi: 10.4049/jimmunol.2000461

Fig. 3. Newborns have higher representation of Tregs in the lung compared to adults, but their phenotype is similar in the two age groups.

Fig. 3.

Lung cells isolated from four newborn (4-5 days of age) and four adult (9.6-12.8 years of age) AGM were evaluated for the presence of Tregs. Cells were initially gated on Zombie excluding (live) CD3+ cells. Tregs within this population were identified by co-staining for FoxP3 and CD25. Data for the percentage of CD3+ cells expressing FoxP3 are shown in A. The level of FoxP3 (B) and CD25 (C) on FoxP3+ cells is shown. The percentage of cells expressing the indicated molecule (top panels) and the level expressed by these cells (bottom panel) for PD-1 (D), ICOS (E), and CD39 (F) was also evaluated. Data from four adults is shown in D-F as one animal had a very low percentage of FoxP3+ cells that did not allow confident analysis of subsets. Individuals animals are indicated; the line represents the average±SEM. Significance was determined using a Student’s t-test. **p<0.005. ns=not significant.