Table 2.

Histological parameters, evaluated using H&E and SR staining

Parameters	Group
	3–4 weeks		9–10 weeks
	Normal diet	CDHFD	Normal diet	CDHFD
Steatosis score	0.0 ± 0.0	3.0 ± 0.0 **	0.0 ± 0.0	3.0 ± 0.0 **
Inflammation score	0.0 ± 0.0	1.4 ± 0.55 **	0.20 ± 0.45	2.6 ± 0.55 **, ††
Ballooning score	0.0 ± 0.0	0.20 ± 0.45	0.0 ± 0.0	1.0 ± 0.0 **, †
NAFLD activity score	0.0 ± 0.0	4.6 ± 0.89 **	0.20 ± 0.45	6.6 ± 0.55 **, ††
Fibrosis area (%)	1.0 ± 0.0	1.4 ± 0.5	1.0 ± 0.0	19.8 ± 10 *, †

Values are expressed as means ± SDs (n = 5). *P < 0.05, **P < 0.01, compared with the respective normal diet-fed groups; ^†P < 0.05; ^††P < 0.01, compared with the 3–4-week CDHFD-fed group

H&E haematoxylin and eosin, SR Sirius red, CDHFD choline-deficient, low-methionine high-fat diet, NAFLD non-alcoholic fatty liver disease