TABLE 2.

Primers used in this study.

Primer	Sequence (5′-3′)	Target gene
FOR qPCR
ECL_7510-5′	ACGCTTGTCACCGGTAAAAC	ECL_RS07510
ECL_7510-3′	TTGATTACCGCACGCATTGG
ECL_7555-5′	TTGCTGTGGTGGATTTGTCG	hcp1
ECL_7555-3′	ACACCGGCTGGACTGATATTAC
ECL_7670-5′	CGCATCGATTTCACGGTTATCC	ECL_RS07670
ECL_7670-3′	TTCACGCGGCCATATTTGTC
ECL_8875-5′	AATGTGACGCTGCGCTTTTC	ECL_RS08875
ECL_8875-3′	AATTACGCATCGCCAGCATG
ECL_8915-5′	TGGGGAGCGTGAAAAATGTG	ECL_RS08915
ECL_8915-3′	ATCATTGCCTGCGGTTTCAG
ECL_8930-5′	TCCCGGGATTAACAGCCTTTC	ECL_RS08930
ECL_8930-3′	TTGCTGCTCCGTTTTCACTG
rrsH-5′	CAGCCACACTGGAACTGAGA	rrsH
rrsH-3′	GTTAGCCGGTGCTTCTTCTG
For cloning
hcp1-HindIII-5′	ATCAAGCTTGAGTAGATATTATGGCTATTG	hcp1
hcp1-BamHI-3′	CCCGGATCCACCCACTACTATTATGCTTCTTTG
clpV1-KpnI-5′	GGGGGTACCTCCTTCCGTATACCGAATCATTGT	clpV1
clpV1-BamHI-3′	GGGGGATCCGTCCGATTGTTATCATTATCCGTCA
clpV2-KpnI-5′	GGGGGTACCCGGGCGATGACCAGTCCAA	clpV2
clpV2-BamHI-3′	GGGGGATCCCACCGAAAGCAGATGATGGC
For mutagenesis
Ecl-clpV1-H1P1	TCATTGTTAATGGAAACTAACATGTCAGAAATTAGCCGTGCCGTGTGTAGGCTGGAGCTGCTTCG	clpV1
Ecl-clpV1-H2P2	TTACAGTTCTCTTGATGATTATTACGCGGCAAACTGACAGTGAAACATATGAATATCCTCCTTAG
Ecl-clpV2-H1P1	GGTGCTGTCGCTTAGGCGCTGGTGGACCGGCAGAGCCTGGTGCGCTGTAGGCTGGAGCTGCTTCG	clpV2
Ecl-clpV2-H2P2	ATCTCAGCGGGGGATTACTGCTCATGCGTGCGCTCCTTCTGTCGACATATGAATATCCTCCTTAG
Ecl-hcp1-H1P1	CATCCACGAAGAGTAGATATTATGGCTATTGATATGTTTCTGAAGTGTAGGCTGGAGCTGCTTCG	hcp1
Ecl-hcp1-H2P2	GCCCTTTCGGACCCACTACTATTATGCTTCTTTGTTTTCTTTGATCATATGAATATCCTCCTTAG

Italic letters indicate the respective restriction enzyme site in the primer. The sequence corresponding to the template plasmid pKD4 is underlined.