FIGURE 4.

ICS after short term stimulation of γδ⁺, CD4⁺, and CD8⁺ T cells in PBMC and LPL with PMA/Ionomycin. (A) (Left) Frequencies of IL-17A⁺ γδ⁺, CD4⁺, and CD8⁺ T cells from healthy donors (green) and patients with IBD (red); (center) Comparison of the frequencies of IL-17A⁺ cells between CD39⁺ and CD39^– γδ⁺ T cells; (right) Comparison of the frequencies of IL-17A⁺ cells between CD39⁺ and CD39^– CD4⁺ T cells; data from PBMC in upper row (triangular shapes), data from LPL in bottom row (round shapes). (B) (Left) Frequencies of IFN-γ⁺ γδ⁺, CD4⁺ and CD8⁺ T cells from healthy donors (green) and patients with IBD (red); (center) Comparison of the frequencies of IFN-γ⁺ cells between CD39⁺ and CD39^– γδ⁺ T cells; (right) Comparison of the frequencies of IFN-γ⁺ between CD39⁺ and CD39^– CD4⁺ T cells; data from PBMC in upper row (triangular shapes), data from LPL in bottom row (round shapes). (C) (Left) Frequencies of IL-10⁺ γδ⁺, CD4⁺ and CD8⁺ T cells from healthy donors (green) and patients with IBD (red); (center) Comparison of the frequencies of IL-10⁺ cells between CD39⁺ and CD39^– γδ⁺ T cells; (right) Comparison of the frequencies of IL-10⁺ cells between CD39⁺ and CD39^– CD4⁺ T cells; data from LPL (round shapes). Data from healthy donor’s LPL (n = 17/12) and PBMC (n = 8) and IBD patients’ LPL (n = 11/9) and PBMC (n = 6), presented as means +/- standard deviation. ns ≥ 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, as calculated by two-way ANOVA and Wilcoxon matched-pairs signed rank test (comparison of CD39⁺ and CD39^– cells). HD, healthy donor; IBD, patient with inflammatory bowel disease; LPL, lamina propria lymphocytes; PBMC, peripheral blood mononuclear cells; ICS, intracellular cytokine staining.