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. 2020 Sep 24;11:581135. doi: 10.3389/fmicb.2020.581135

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Copyright © 2020 Sexton and Tocheva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cryo-ET of vegetative, sporulating, and germinating Streptomyces. Tomographic slices through S. albus at different growth stages corresponding to (A) vegetative hyphae, (B) aerial hyphae formation during early sporulation, (C) septa formation during early sporulation, (D) spore maturation, (E) release of mature spores, and (F) germination. Cytoplasmic membrane (CM) is shown in red. Vegetative peptidoglycan (PG) and spore wall (SW) are shown in green. Rodlet ultrastructure and spore coat are shown in pink and dark purple, respectively. Each tomographic slice is 20-nm thick. Scale bar = 200 nm.