FIGURE 1.

Differential change in biomarkers between anemic and non-anemic patients. A Heatmap was designed to depict the overall pattern of complete blood counts (CBC) (A) and biochemical markers (B) at all timepoints in anemic and non-anemic at different study timepoints of anti-tubercular treatment. A two-way hierarchical cluster analysis (Ward’s method) was performed. Dendrograms represent Euclidean distance. Expression scale represents Z-score normalization from the median at each timepoint and group. To define anemia according to baseline hemoglobin, the cut-off point of 12 g/dL for women and 13.5 g/dL for men was used. A log₁₀ of fold-change was calculated and statistical analyses were performed using the Mann–Whitney U adjusted test. Significative differences (p < 0.05) between anemic and non-anemic patients for each time point are highlighted in red bars. Data are from 191 patients who had complete information on cell counts and biochemical measurements at all study timepoints.