FIGURE 4.

Patients who persists with anemia exhibit substantially higher degree of inflammatory perturbation. (A) Degree of inflammatory perturbation (DIP) is based on Molecular Degree of Perturbation (16), but instead of using gene expression, we use biochemical and cellular markers. DIP was calculated was calculated using the median and standard deviation of the control group as a starting point. Then, the Z-score was calculated for all groups, a cut-off point was established and, finally, an average disturbance calculation was performed for each sample. This figure is adapted https://mdp.sysbio.tools/about. (B,C) Left panels: Histograms show the single sample degree of inflammatory perturbation (DIP) score values relative to the non-anemic at baseline group between patients recovered from anemia and with persistent anemia at baseline (B) and day 180 (C). Right panels: Scatter plots of the summary data for each group are shown. DIP score values were compared between non-anemic at baseline (control group), recovered from anemia and persistent anemia at baseline (B) and day 180 (C). Lines in the scatter plots represent median values. Data were compared using the Mann–Whitney U test. *P < 0.05; ***P < 0.0001. Data are from 191 patients who had complete information on cell counts and biochemical measurements at all study timepoints.