Figure 2.

Effect of Hst and Dox on Cell Cycle and HER2 Expression of MCF-7/HER2. MCF-7/HER2 was seeded into a 6-well plate, added with Hst Dox, and the Combination and Incubated for 24 h. Cells were harvested and added with PI reagent and analyzed using a flow cytometer. (A), Cell distribution profile of (i) cell control (ii) Dox 0.2 μM (iii) Hst 95 μM dan (iv) combination Hst and Dox; (B), Percentage of the cell population in each phase after various treatments; (C), HER2 expression was examined using western blot. Band visualization was obtained using Luminograph; (D), Band obtained were quantified using ImageJ software showed an intensity ratio of HER2 and ß-actin after treatment with Hst, Dox, and the combination showed that the treatment decreased HER2 expression