Figure 2.

Quantification of total neurite length of DRG primary sensory neurons treated in vitro with the vehicle (CTR) or with the following VCR concentrations: 0.01, 0.1, 1, 5, 10, 50, and 100 nM and 1, 5, 10, 20, 50, and 100 μM. In the same figure are reported representative images of β3-tubulin (red) staining in DRG cell cultures. Cell nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. Data are presented as mean ± SEM, n ≥ 80 cells obtained from four independent experiments. One-way ANOVA followed by Bonferroni's post-test. ***p < 0.001 vs. CTR; ^°°°p < 0.001 vs. VCR 0.01 nM; ###p < 0.001 vs. VCR 0.1 nM; +p < 0.05, ++p < 0.01, +++p < 0.001 vs. VCR 1 nM.