Table 5.

The SCEs per chromosome and sister chromatid exchanges (SCEs) per cell in human lymphocytes treated with cyclophosphamide (CPA) alone, ethanol extract of Andrographis paniculata (EAP) alone and CPA+EAP after 48 h treatment

Treatment	No. of metaphases scored	Total no. of SCEs scored	Mean SCEs / chromosome	SCEs/cell (mean±SE)
Normal control	50	127	0.0552	2.54±0.12
CPA (100 μg/mL)	50	496	0.2156**	9.92±0.63**
EAP 50 mg/mL	50	120	0.0521	2.40±0.08
EAP 100 mg/mL	50	124	0.0539	2.48±0.06
EAP 150 mg/mL	50	119	0.0508	2.34±0.06
EAP 200 mg/mL	50	126	0.0547	2.52±0.11
EAP 250 mg/mL	50	131	0.0569	2.62±0.05
CPA+EAP 50 mg/mL	50	419	0.1821*#	8.38±0.14*#
CPA+EAP 100 mg/mL	50	376	0.1634*##	7.52±0.11*##
CPA+EAP 150 mg/mL	50	310	0.1347**##	6.20±0.13**##
CPA+EAP 200 mg/mL	50	274	0.1191**##	5.48±0.20**##
CPA+EAP 250 mg/mL	50	228	0.0991**##	4.56±0.18**##

Significantly different from normal control at *P<0.05 and **P<0.01, and positive control (CPA) at ^#P<0.05 and ^##P<0.01.

A total 50 well spread metaphases/cultures were scored for the occurrence of SCE, no. of donors=2.