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Transcriptional level of Zot mRNA during course of infection and cytotoxicity. (A) Zot expression levels of PMC53.7 strain and cytotoxicity (%) during the course of infection of Caco-2 cells. * Up-regulated vs rpoS (reference gene) (B) Cytotoxicity (%) of Caco-2 cells induced by treatment with 100 μg of purified PMC53.7-Zot and E. coli proteins (EP) control. All the experiments were done in triplicate and repeated three times.
