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. 2020 Jun 27;19(4):357–364. doi: 10.1002/rmb2.12335

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© 2020 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Representative images of oocytes or embryos in this study. A, Fresh oocyte. B, 1‐day‐old oocyte. C, Reconstructed oocyte from FF group (cytoplast from fresh oocyte and spindle from another fresh oocyte). D, Reconstructed oocyte from FO group (cytoplast from 1‐day‐old oocyte and spindle from fresh oocyte). E, Most of the reconstructed oocytes from FO group had fragmentation. F, Reconstructed oocyte from OF group (cytoplast from fresh oocyte and spindle from 1‐day‐old oocyte). G, Embryo in the two‐cell stage from OF group. H, Embryos in the blastocyst stage from OF group