Table 2.
Results of experiment 2, metaphase II spindle transfer (MESI), and intracytoplasmic sperm injection (ICSI), for the three groups of FF, FO, and OF
| N | No. surviving after MESI (% of N) | No. of fragments after MESI (%) | No. surviving after ICSI (%) | No. of fragments after ICSI (%) | No. of PN (%) | No. of. embryos developing to | ||
|---|---|---|---|---|---|---|---|---|
| 2‐cell stage (% of PN) | Blastocyst stage (% of PN) | |||||||
| FF | 334 | 231 (69.2) | 5 (2.2)a | 156 (67.5) | 13 (8.3)c | 143 (91.7) | 133 (93.0) | 72 (50.3)e |
| FO | 393 | 155 (39.4) | 88 (56.8)b | 63 (40.6) | 34 (54.0)d | 29 (46.0) | 12 (41.4) | 0 (0)f |
| OF | 402 | 223 (55.5) | 13 (5.8)a | 147 (65.9) | 42 (28.6)c | 105 (71.4) | 87 (82.9) | 32 (30.5)e |
Abbreviations: FF, cytoplast from fresh oocyte and spindle from another fresh oocyte; FO, cytoplast from 1‐day‐old oocyte and spindle from fresh oocyte; ICSI, intracytoplasmic sperm injection; MESI, metaphase II spindle injection; OF, cytoplast from fresh oocyte and spindle from 1‐day‐old oocyte; PN, pronuclear.
a vs. b: P < .01, c vs. d: P < .01, e vs. f: P < .01