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Comparison of the amplification method on the sensitivity of barley yellow dwarf virus (BYDV) detection. (A) Reverse transcription recombinase polymerase amplification (RT-RPA). (B) Reverse transcription polymerase chain reaction (RT-PCR). Amplified products (182 bp from the RT-RPA assay and 831 bp from RT-PCR) were visualized on 1.2% agarose gels. M, DNA marker; lanes 1-8, 10-fold serial dilution (100-10-7) of BYDV template (coat protein transcripts).
