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Specificity of the reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of barley yellow dwarf virus (BYDV). RT-PCR, reverse transcription polymerase chain reaction; M, DNA marker; lanes 1-4, RT-RPA product amplified from BYDV-, barley mild mosaic virus (BaMMV), and barley yellow mosaic virus (BaYMV)-infected (lanes 1-3) and healthy (lane 4) tissues, respectively; lane 5, negative control. An actin was used as an internal control.
