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. 2020 Oct 6;13:40. doi: 10.1186/s13072-020-00362-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — SETD2 is robustly degraded by the proteasome. a Western blot of whole-cell lysates probed with the depicted antibodies. Lysates of wild type 293T (untransfected) cells or expressing SETD2 full-length (FL) were prepared after 12 h of MG132 (10 µM) treatment. The expected band for the target protein is indicated by an arrow. b Microscopy images showing the effect of MG132 treatment on expression of GFP-SETD2 FL in 293T cells. The scale bar is 1 mm. c RNA was isolated from GFP-SETD2 FL transfected cells and RT-PCR was performed to check the transcript levels. GAPDH was used as a normalization control. VC- empty vector control. d Whole-cell lysates of 293T cells were prepared after 12 h of MG132 (10 µM) treatment and probed with the antibodies depicted. e Microscopy images showing the effect of MG132 treatment on the expression of GFP-SETD2 FL in the cell lines described. The scale bar is 1 mm