Table 2.

Mobile phase gradient

Time (min)	Flow Rate (ml/min)	%B
0	0.3	0
8	0.3	0
10	0.3	3
28	0.3	10
35	0.3	15
43	0.3	20
48	0.3	25
55	0.3	40
62	0.3	60
68	0.3	95
73	0.3	95
73.1	0.3	0
76	0.3	0