Fig. 3.

BDNF increases protein expression of glutamate transporters. Cortical (a) and striatal (b) astrocytes were incubated for 24 h with 3NP 15 mM with or without BDNF 50 ng/ml and GLT1 protein levels in total cell protein extracts were determined by Western blot. Values represent arbitrary units of the GLT1/GAPDH ratio ± SEM of n = 5 independent experiments for cortical and 4 independent experiments for striatal astrocytes. The effect of both factors BDNF and 3NP was analyzed by two-way ANOVA *p < 0.05 and ***p < 0.001 vs. control group ^p < 0.05 and ^^^p < 0.001 vs. 3NP group. Cortical (c) and striatal (d) astrocytes were incubated for 24 h with 3NP 15 mM with or without BDNF 50 ng/ml and GLAST protein levels in total cell proteins extracts were determined by Western blot. The values represent arbitrary units of the GLAST/GAPDH ratio ± SEM of n = 4 independent experiments for cortical and n = 5 independent experiments for striatal astrocytes. The effect of both factors BDNF and 3NP was analyzed by two-way ANOVA. ***p < 0.001 vs. control group