Fig. 5.

BDNF protects ST14A/Q120 neurons from 3NP-induced cell death. a TrkB-FL and β3-tubulin protein levels in total cell protein extracts of Q15 or Q120 cells in control conditions were determined by Western blot. Image shows one representative blot out of two. Q15 (b) and Q120 (c) cells were incubated with 3NP 10 mM with or without BDNF 100 ng/ml for 24 h and cell viability was evaluated by Trypan blue exclusion assay. Values are the mean ± SEM of viable cells/ml of n = 3 independent experiments. The effect of both factors BDNF and 3NP was analyzed by two-way ANOVA. *p < 0.05, ***p < 0.001 vs. control group and ^p < 0.05 vs. 3NP group