Fig. 8.

Effects of paclitaxel (pac) and cisplatin (cis) on the expression of chemoresistant genes (ERCC1 and TUBB3), and cancer stem cell markers (CD44, Oct4A, CD133 and EpCAM) in OVCAR4 Cont and T2-KD cells. The mRNA and protein expression of a ERCC1 (red) and TUBB3 (green), b CD44 (green), Oct4A (green), CD133 (green) and EpCAM (red), and c P-STAT3 (red) and T-STAT3 (red) in OVCAR4 Cont and T2-KD OVCAR4 cells treated with their respective IC₅₀ doses of cis and pac were determined by immunofluorescence and relative qRT-PCR (untreated cells grey, cis treated red and pac treated, blue). Each experiment was repeated three times and was performed in triplicate. For immunofluorescence images, magnification was (20X); scale bar (in yellow) 20 μM. Significance was determined by one-way ANOVA *p > 0.05; **p > 0.01; ***p > 0.001; ****p < 0.0001 in Cont vs T2-KD cells