Table 3.

Primer sequences, accession numbers, fluorescence capture of the genes analysed

Name	Primer sequences (5' - 3')	Accession number	Fluorescence capture (°C)
18S rRNA	F GTAACCCGTTGAACCCCATT R CCATCCAATCGGTAGTAGCG	NR_003286.1	78
TIMP-1	F TGACATCCGGTTCGTCTACA R GTTTGCAGGGGATGGATAAA	NM_003254.2	85
TIMP-2	F CCGCAACAGGCGTTTTGCAA R TCACTTCTCTTGATGCAGGC	NM_003255.4	85
TIMP-3	F TTCTGCAACTCCGACATCGT R ATGCAGGCGTAGTGTTTGGA	NM_000362.4	83
ERCC1	F TTGTCCAGGTGGATGTGAAA R GCTGGTTTCTGCTCATAGGC	NM_202001.2	83
TUBB3	F GGCCTTTGGACATCTCTTCA R ATACTCCTCACGCACCTTGC	NM_006086.3	88
CD44	F CCAATGCCTTTGATGGACCA R TGTGAGTGTCCATCTGATTC	NM_000610.3	81
OCT4A	F CTCCTGGAGGGCCAGGAATC R CCACATCGGCCTGTGTATAT	NM_002701.4	88
PROM1/ CD133	F ATTGGCATCTTCTATGGTTT R GCCTTGTCCTTGGTAGTGT	NM_006017	78
EpCAM	F CGTCAATGCCAGTGTACTTCAGTTG R TCCAGTAGGTTCTCACTCGCTCAG	NM_002354.2	80
MMP14/ MT1-MMP	F GCTCCGAGGGGAGATGTTTG R CAGCTCCTTAATGTGCTTGGG	NM_002428	83
MMP-2	F TTGACGGTAAGGACGGACTC R ACTTGCAGTACTCCCCATCG	NM_004530.4	81
E-CAD/ CDH1	F GGCACAGATGGTGTGATTACAG R GTCCCAGGCGTAGACCAAGAAA	NM_004360.3	75
N-CAD/ CDH2	F AAACAGCAACGACGGGTTAG R CTTAGGATTGGGGGCAAAAT	NM_001792.3	78
VIM	F CCTACAGGAAGCTGCTGGAA R GGTCATCGTGATGCTGAGAA	NM_003380.3	75
COL12A1	F ACCTGTCACTGTTCGGGAAG R TGAGGGAAGTGCTGGTCTCT	NM_080645.2	80