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. 2020 Oct 7;10:16749. doi: 10.1038/s41598-020-74021-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MCARS analysis of CRC cell lines. MCARS spectral images of living HCT116, HT29 and SW620 cells including bright-field, and fluorescence (Hoechst 33342) images. MCARS images were reconstructed from signal integration at 2850 cm⁻¹ (CH₂ symmetric stretching) and 2930 cm⁻¹ (CH₃ symmetric stretching) (N = 3). Scale bar, 5 µm.