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. 2020 Oct 7;10:16749. doi: 10.1038/s41598-020-74021-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MCARS spectroscopy of HT29 cells incubated with K252a. HT29 cell lines were treated with K252a (100 nM), during 48 and 72 h. From top to bottom: bright-field, fluorescence (Hoechst 33342) images, MCARS images reconstructed from signal integration at 2850 cm⁻¹ (CH₂ symmetric stretching) and 2930 cm⁻¹ (CH₃ symmetric stretching), merged images (N = 2). Scale bar, 5 µm.