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. 2020 Oct 7;11(10):832. doi: 10.1038/s41419-020-2584-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a RT-qPCR data of LL22NC03-N14H11.1 level in SK-HEP-1 and Huh7 cells transfected with sh-NC or sh-LL22NC03-N14H11.1#1/2/3. b Cell viability of SK-HEP-1 and Huh7 cells at indicated time was evaluated by CCK-8 under indicated transfection. c Representative images and quantification of colonies formed by two HCC cells under indicated transfection. d Flow cytometry analysis of apoptotic HCC cells under LL22NC03-N14H11.1 silence vs. control. e Western blots of cleaved and total caspase-3 and caspase-6, Bax, and Bcl-2 in 2 HCC cells. f Representative pictures of invading HCC cells and quantification under LL22NC03-N14H11.1 depletion were obtained via transwell assay. Scale bar: 100 μm. g Images of wound width of HCC cells of each group and quantification of relative distance of wound healing obtained from wound healing assay. h Western blots of E-cadherin, N-cadherin, MMP2, and MMP7 in HCC cells with LL22NC03-N14H11.1 silence. i IF staining of E-cadherin and N-cadherin in HCC cells of each group. Scale bar: 50 μm. **P < 0.01.