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. 2020 Oct 7;11(10):832. doi: 10.1038/s41419-020-2584-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Western blot results of PDI1, CDK1, p-ERK1/2, and total ERK1/2 in HCC cells under LL22NC03-N14H11.1 silence. b Western blot and RT-qPCR detected the expression of H-RAS (G12V) in Huh7 and SK-HEP-1 cells under LL22NC03-N14H11.1 silence. c Cycloheximide (CHX) was added to inhibit protein generation. Protein level of H-RAS (G12V) in SK-HEP-1 and Huh7 cells under LL22NC03-N14H11.1 silence was detected by western blot and quantified at 0, 3, 6, and 9 h after CHX treatment. d CoIP followed by western blot analyzed the levels of H-RAS (G12V) and LZTR1 in the binding complex of LZTR1 and control IgG, as well as their levels in input. e CoIP assay determined the ubiquitination level of H-RAS (G12V) in SK-HEP-1 cells under LL22NC03-N14H11.1 silence with or without MG132 treatment, and the level of input H-RAS (G12V) and LZTR1 was also detected via western blot. f Western blots of H-RAS (G12V) and LZTR1 in HCC cells under LL22NC03-N14H11.1 silence. g RT-qPCR analysis of LZTR1 level in HCC cells under LL22NC03-N14H11.1 silence. h RT-qPCR analysis of LL22NC03-N14H11.1 level in HCC cells under LZTR1 silence. i RT-qPCR analysis of LZTR1 level in 62 pairs of HCC tissues and matched non-tumorous tissues. j Negative correlation between LZTR1 and LL22NC03-N14H11.1 in HCC tissues was confirmed by Pearson’s correlation curve. k RT-qPCR analysis of LZTR1 in HCC cell lines vs. normal cell line. **P < 0.01; n.s. no significance.