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. 2020 Oct 7;11(10):832. doi: 10.1038/s41419-020-2584-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Luciferase activity of LZTR1 promoter reporter in HCC cells under LL22NC03-N14H11.1 silence. b Ten transcription factors potentially targeting LZTR1 promoter was predicted by human TFDB (P < 0.001) and PROMO (P < 0.05). c The enrichment of ten predicted TFs in the pulldown of LL22NC03-N14H11.1 biotin vs. LL22NC03-N14H11.1 no biotin in HCC cells was quantified. d RT-qPCR assessed the expression of LZTR1 at mRNA and protein levels in HCC cells under the silence of ELF1, AR, and c-Myb, e RT-qPCR analysis of LL22NC03-N14H11.1 enrichment in precipitates of c-Myb after RIP assay. Interaction between U1 and SNRNP70 served as the positive control of this assay. f FISH staining of LL22NC03-N14H11.1 and IF analysis of c-Myb showed their co-localization in the nucleus of SK-HEP-1 cells. Scale bar: 25 μm. g Three predicted c-Myb sites on LZTR1 promoter. h Luciferase activity of wild-type (WT) LZTR1 promoter reporter or with site 1/2/3 mutated, respectively, in 293T cells under c-Myb overexpression. i RT-qPCR analysis of LZTR1 promoter with predicted c-Myb site 1, 2, or 3 in the RIP of c-Myb in HCC cells. j Enrichment of LZTR1 promoter in the ChIP complexes of c-Myb under LL22NC03-N14H11.1 silence vs. control. k HCC cells were transfected with sh-NC, sh-LL22NC03-N14H11.1#1, sh-LL22NC03-N14H11.1#1 + pcDNA3.1, or sh-LL22NC03-N14H11.1#1 + pcDNA3.1/c-Myb, respectively. RT-qPCR data of LZTR1 and c-Myb levels in HCC cells of each group. l Western blot results of LZTR1, c-Myb. H-RAS (G12V), p-ERK1/2, total ERK1/2, p-DRP1 (S616) and total DRP1 in HCC cells of each group. **P < 0.01; n.s. no significance.