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. 2020 Oct 7;11:5035. doi: 10.1038/s41467-020-18833-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Construction of E. coli cells expressing P450_BM3 variants (A82F, A82F/A328F and 19A12) and glucose dehydrogenase (GDH). See Supplementary Fig. 6 for SDS-PAGE of whole-cell proteins of module 1 expressed in E. coli. RSF: pRSFDuet-1; arrow: T7 promoter. Red filled rectangle: P450_BM3 variants genes; blue filled rectangle: GDH gene. b Engineered E. coli cells containing enzyme module 1 (P450_BM3 variants) for the biotransformation of CH 1b to CHOL 2b. Purple column: CHOL; yellow column: CHONE. The data shown in b are presented as mean value ± SD (standard deviations) of three biological replicates. Source data are provided as a Source Data file.