Table 1.

One-pot DCAs production with designed E. coli consortia.

Entry	Substrates	Conc. of DCAsa (mM)	Product distribution [%]b
			2a-d	3a-d	4a-d	5a-d	7a-d
1c	n = 1, 2a	48	0	7	0	0	93
2c	n = 2, 2b	46	0	9	0	0	91
3c	n = 3, 2c	49	0	0	0	0	>99
4c	n = 4, 2d	42	0	0	0	7	93
5d	n = 1, 1a	12	0	0	0	0	>99
6d	n = 2, 1b	21	0	0	0	0	>99
7d	n = 3, 1c	20	0	0	0	0	>99
8d	n = 4, 1d	6.1	17	13	0	17	53

^aDetermined by gas chromatography.

^bRelative amounts based on the concentrations of dicarboxylic acids 7a-d and analyzed by gas chromatography. Data are presented as mean value of two biological replicates. Source data are provided as a Source Data file.

^cReactions were conducted with indicated substrates (50 mM) in 4 mL cell suspensions at 16 g CDW L⁻¹. EC2_3 containing E. coli (M2E) and E. coli (M3B_M3E) at a ratio of 2:1 was in 200 mM KP buffer (pH 8.0) at 25 °C and 200 rpm for 24 h.

^dReactions were conducted with indicated substrates (50 mM) in 4 mL cell suspension at 12 g CDW L⁻¹. EC1_2_3 containing E. coli (M1E), E. coli (M2E), and E. coli (M3B_M3E) at ratio 2:1:2 was in 200 mM KP buffer (pH 8.0) with 0.05 g mL⁻¹ glucose at 25 °C and 200 rpm for 24 h.