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. 2020 Oct 7;10:16756. doi: 10.1038/s41598-020-73802-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical staining for fibrillin-1 on CM cell culture for corrected and MFS. Fibrillin-1 in red and nuclei stained with HOECHST in blue after 25 days after the start of cardiac differentiation. Fibrillin-1 microfibrils or punctate assemblies are observed in corrected or MFS cultures, respectively. The average fluorescent intensity of fibrillin-1 in this image is respectively 26.65 and 19.51 for corrected and MFS cell culture. Orthogonal view shows the z-section of the area, indicated with the arrow in the overlay image. Bar indicates 100 µm.