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. 2020 Oct 7;10:16756. doi: 10.1038/s41598-020-73802-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MEA measurements of the extracellular field potentials for the control line (H9), the corrected CMs and the MFS CMs after 20–25 days after start of cardiac differentiation. (A) The baseline measurements without the addition of isoproterenol (ISO). The extracellular field potentials are shown for 10 nM (B) and 100 nM ISO (C). The bar plots show the differences between control, corrected and MFS CMs for RR interval (D), SDNN (E), SDSD (F) and coefficient of variance (G). Corrected CMs (n = 9, black), MFS CMs (n = 8, red). Poincaré plot from three representative replicates of the control (blue), corrected (black) and MFS CMs (red) (H). The three clusters of MFS are magnified in the right corner of figure (H). The level of significance is indicated by asterisks: p values less than 0.001 are indicated with three asterisks and p values less than 0.01 indicated with two asterisks.